Diabetic rats were treated with pyridoxamine 10 and 15 mg per kg body weight and compared with a control set of diabetic rats without supplementation. Leaf extracts in alloxan induced diabetic rats syed mansoor ahmed, vrushabendra swamy bm, p gopkumar r dhanapal and vm chandrashekara department of pharmacology, m. This is attributed to break down of adipose tissue lipids and skeletal muscle protein. Pdf silymarin increases antioxidant enzymes in alloxan. The effect of vernonia amygdalina on alloxan induced diabetic. Aug 22, 2019 in the present study, there was a significant decrease in cat activity in the diabetic group compared to the control group which was in line with oseni, odesanmi, and oladele who reported a decrease in plasma cat activity in alloxan induced diabetic male wistar albino rats due to increased production of ros and the reduction in the synthesis of.
Diabetes was induced by administration of 120 mgkg alloxan monohydrate. The compound has the molecular formulae, c 4 h 2 n 2 o 4 and a relative molecular mass of 142. The aim of this study was to investigate the protective effect of cordymin on diabetic osteopenia in alloxan induced diabetic rats and the possible mechanisms involved. Furthermore, alloxan inhibits glucokinase, a shcontaining protein essential for insulin secretion induced by glucose. In vitro and in vivo antidiabetic activity of the leaves. Alloxaninduced diabetes delays repair in a rat model of closed tibial. Research article alloxan induced diabetes mellitus in wistar. Decreased body weight is commonly observed in alloxan induced diabetic rodents. Nutrients free fulltext nigerian honey ameliorates. The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxan induced diabetic male rats.
Aug 24, 2017 the study revealed significant increase in blood glucose level was observed in alloxan induced diabetic rats. Antidiabetic activity of gossypium herbaceum by alloxan. Comparison of streptozotocin and alloxan induced diabetes in the rat, including volumetric quantitation of the pancreatic islets, volume 9, issue3, pp 178184 cite as comparison of streptozotocin and alloxan induced diabetes in the rat, including volumetric quantitation of the pancreatic islets diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. The asparagus racemosus was evaluated for its antidiabetic activities and hypolipidemic activity against alloxan induced hyperglycemia in rats. Oxidative stress and redox modulation potential in type 1 diabetes oxidative stress and redox modulation potential in type 1 diabetes. Hypoglycemic and hyperinsulinemic effects of ferula. Comparison of streptozotocin and alloxaninduced diabetes.
The mechanisms of alloxan and streptozotocininduced diabetes. Comparative effects of rothmannia hispida leaves extract and. Alloxan definition of alloxan by medical dictionary. Iv, alloxan induced diabetic rats and was treated with a low dose of bv at a daily dose 1 mgkg for six weeks. Diabetic rat treated with aqueous extract of garcinia kola.
Thirty 30 adults male wistar rats were randomly divided into six 6 groups of five 5 each based on their body weight. The responses are accompanied by corresponding inverse changes in. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Antidiabetic effect of ipomoea batatas in normal and alloxan. Alloxan which is chemically known as 5,5dihydroxyl pyrimidine2,4,6trione is an organic compound, a urea derivative, a carcinogen and cytotoxic glucose analog. Alloxan diabetic rats were orally supplied with kombucha and black tea at a dose of 5 mlkg body weight per day for 30 days, fasted overnight. Effect of crude oil pollution on phytochemical and anti.
Groups 2 4 in additional to control diet received alloxan treatment 150mgkg i. Introduction diabetes mellitus is a group of metabolic disorders that result in. The asparagus racemosus was evaluated for its antidiabetic activities against alloxan induced hyperglycemia in rats. We read with interest the article titled anomalies in alloxaninduced diabetic model. The non diabetic induced rats exhibited steady increase 8. Hypoglycaemic and antidiabetic activity of stem bark extracts. Hepatoprotective and hypolipidemic effects of carthamus tinctorius linn. Hypoglycemic and antioxidant effects of leaf essential oil. Alloxan induced diabetic model in rats to investigate the hypoglycemic theffect of the ethylether and ethanol extracts, the fasted rats were divided into 5 groups of 6 each. It has been successfully induced in a variety of animal species. Pdf antidiabetic activity of extract on alloxan induced diabetic rats. Sensitization to alloxan induced diabetes and pancreatic cell apopto. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat.
Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Formation of aunps were confirmed by uvvis spectroscopy, fourier transformed infrared ftir spectroscopy. Effect of 30 day administration of prebiotic beverage on alloxan induced diabetic rat on the following parameters a catalase activity u. Jun 26, 2012 hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxan induced diabetic rats. Pdf estimation of glucose level and body weight in alloxan. Honey treatment did not improve % change in body weight in diabetic rats. A single dose of alloxan monohydrate 100 mgkg was given intravenously to 20 young new zealand white rabbits. Thirty rats were used divided into 2 groups, normal control. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Diabetes was induced by the single intra peritoneal dose of alloxan 120 mg per kg body weight.
Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan induced diabetic rats. Indeed, in rat islets incubated with alloxan the gsh content and gshgssg ratio were decreased malaisse et al. Hyperglycaemic rats were treated with or without oral. Antidiabetic activity of methanol leaf extract of costus. Dose optimization of alloxan for diabetes in albin. Effects of diabetes mellitus induced by alloxan on the. Indeed, the essential oil of rosescented geranium leaves was prepared and their antioxidant activities as well as their hypoglycemic effects were studied. Antihyperglycemic and antihyperlipidemic effect of.
The present study is the preliminary assessment of the antidiabetic activity of the ethanolic and the aqueous grain extracts of paspalum scrobiculatum. C fruit powder on serum glucose level and body weights in alloxan induced diabetic rats. Jayshree department of pharmacognosy, college of pharmacy, madras medical college, chennai600 003, india. It is used as a cleansing and purifying agent and yet can be considered as a bodybuilder because of its nutritional value. Alloxaninduced diabetes triggers the development of. Biosynthesis of gold nanoparticles aunps using sargassum swartzii and its anti diabetic effect were studied using male wistar albino rats. This study was to create a longterm rabbit model of diabetes mellitus for medical studies of up to one year or longer and to evaluate the effects of chronic hyperglycemia on damage of major organs.
Group v, alloxan induced diabetic rats and was treated with vildagliptin galvus 50 at a daily dose 10 mgkg akarte et al. The prime focus of this study is to estimate the potential of ipomoea batatas l peeloff on magnesium and calcium level in alloxan induced diabetic rats. Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. This study reports the effects of pyridoxamine supplementation in alloxan induced diabetic rats. Permanent diabetes can be induced reproducibly in the rat, mouse, dog, and rabbit, although some species appear resistant, including the cat, guinea pig, and pig. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. Some antidiabetic properties of prosopis farcta extracts in.
The seed oil of safflower at dose of 200 mgkg was administered as single dose per day to diabetic rats for a period of 28. Sarojini ramulamma college of pharmacy, seshadrinagar, mahabubnagar, andhra pradesh, india keywords. Safflower seed oil was investigated in diabetic rats. Antidiabetic effect of the egyptian honey bee apis. Antidiabetic activity of vinca rosea extracts in alloxan. Alloxaninduced diabetes, a common model for evaluating the. To study the effect of shilajit a herbomineral preparation on blood glucose and lipid profile in euglycemic and alloxan induced diabetic rats and its effects on the above parameters in. Alloxan 150mgkg was used to induce diabetes in rats and the blood glucose levels were estimated by using glucometer. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. The study found that alloxan induced diabetic rats exhibited significant % reduction in body weight. Alloxan induces diabetes by pancreatic cell damage mediated through generation of cytotoxic oxygen free radicals. The mechanism of alloxan and streptozotocin action in b cells. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. The root extract was the best one for reduction of serum glucose followed by leaves then fruits.
Alloxan induced insulin release is, however, of short duration and is followed by complete suppression of the islet response to glucose, even when high concentrations 16. Antidiabetic activity of extract on alloxan induced diabetic rats. Alloxaninduced diabetes, a common model for evaluating. Ferula assafoetida on carbohydrate metabolism in alloxaninduced diabetes. Different doses of ethanolic fraction of ribes nigrum fruit were evaluated for. The present study aimed to investigate and compare the hypoglycemic and antilipidemic effects of kombucha and black tea, two natural drinks commonly consumed around the world, in surviving diabetic rats.
Vernonia amygdalina leaf extract, alloxaninduced diabetic rats. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic. Alloxan induced diabetic rats model was used for this preclinical study. Yxk12 is a commercially available polyherbal product recently introduced in the market and manufacturers claim the product to have anti diabetic properties. Introduction diabetes is a very popular disease that cuts across. In research it is used for induction of type 1 diabetes. Blood glucose level and lipid profile of alloxaninduced. Jun 22, 2005 the different doses of diasulin on blood glucose and plasma insulin in diabetic rats were studied and the levels of lipid peroxides tbars, and hydroperoxide and tissue lipids cholesterol, triglyceride, phospholipides and free fatty acids were also estimated in alloxan induced diabetic rats. Effects of andiroba oil carapa guianensis on wound. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as. Hepatoprotective and hypolipidemic effects of carthamus. Hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxan induced diabetic rats. Composed from 10 rats and diabetic group composed from 20 rats.
The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxan induced diabetic rats. Effect of shilajit on blood glucose and lipid profile in. Antidiabetic activity of paspalum scrobiculatum linn. Silymarin increases antioxidant enzymes in alloxan induced diabetes in rat pancreas. Diabetes was induced in rats by administration of alloxan monohydrate. Hypoglycemic and antilipidemic properties of kombucha tea. In the present study, alloxan induced diabetic rats showed significant p diabetic control rats were higher than those of normal rats. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. Antihyperglycemic potential of teak seeds in alloxan induced diabetic rats html full text. Antioxidant and prebiotic effects of a beverage composed.
Moringa oleifera leaf extract ameliorates alloxaninduced. Andiroba oil on wound healing in rats with induced diabetes. The effects of dosage and the routes of administrations of. Analysis of certain biochemical indices on alloxan induced. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. This causes an insulindependent diabetes mellitus called alloxan diabetes in these animals, with characteristics similar to type 1 diabetes in humans. The herbal formulation known as herbamed was administered to diabetic rats for 7 days at 0. Phytochemical composition of the herbal extracts, fasting plasma glucose concentration fpgc, and serum. Group i normal control rats were received 1% vv tween 80 group ii diabetic control rats were administered alloxan 150 mgkg, i. It also increased live body weight gain and normalized the. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. In the guinea pig, administration of alloxan causes either transient or no hyperglycemia, a reduction in insulinpositive cells in the islets, and a reduction in serum insulin. Alloxan was administered as a single dose 120 mgkg bw to induce diabetes.
Cordymin could restore the circulating blood glucose, glycosylated hemoglobin hba1c, serum alkaline. It was revealed that alloxan induced rats treated with 200mgkgday, 300mgkgday and 400mgkgday sustained percentage weight loss of 48. Alloxan is a urea derivative which causes selective necrosis of the. Hypoglycemic and antilipidemic properties of kombucha tea in alloxan induced diabetic rats. This study evaluated the longterm effects of alloxan induced diabetes in rat liver.
African locust bean, a natural nutritional condiment that features frequently in some african diets as a spice, was investigated in the present study in alloxan. Alloxan diabetes definition of alloxan diabetes by medical. Hypoglycemic activity of aqueous root bark extract zanthoxylum. The animals were distributed into 4 groups of 5 rats each namely. Antidiabetic and antihyperlipidaemic activity of ethanol. In vitro and in vivo antidiabetic activity of the leaves of ravenala madagascariensis sonn. To evaluate the antidiabetic activity of ethanolic extract of dioscorea alata in glucose loaded and alloxan induced diabetic rats. The approach and novelty of this scientific work was to formulate the appropriate streptozotocin stz and alloxan dosage in different routes of administration to imply minimum mortality rate and high incidence of diabetes mellitus dm in the rat experiment model.
Overall, our data demonstrate that hse possesses strong hypolipidemic as well as antioxidant properties in alloxan. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. In alloxaninduced diabetic rats, both the extracts decreased blood sugar levels with significant improvement in glucose tolerance and body weight at the end of. The mechanisms of alloxan and streptozotocin induced diabetes. It is better to standardize it first by jain et al. Another 12 agematched normal rabbits were used as controls. Objectives this study is aimed at investigating the effect of crude oil pollution on the phytochemical content and anti diabetic potentials of oxytenanthera abyssinica rhizomes in normal and alloxan induced diabetic rats using standard methods. In the in vivo experiment, glucose given to rats 20 min prior to alloxan partially restricted alloxan induced increase in the activity of glutathione peroxidase.
Group a served as the control and received 1 mlkg body weight of 5% ethanol being solvent used, group b received 1 ml alloxan. Hypoglycaemic activity of a polyherbal product in alloxan. The hypoglycaemic effect of fermented seeds of parkia biglobosa pb. Alloxan cause immense reduction in insulin release by the destruction of beta cells of the islets of langerhans and caused hyperglycemia. Pdf effect of biosynthesized gold nanoparticles by. Most studies have shown that alloxan is not toxic to the human betacell, even in very high doses, probably because of differing glucose uptake mechanisms in humans and rodents. Antidiabetic activity of ethanolic extract of tubers of. Studies on the administration of vernonia amygdalina del. Estimation of glucose level and body weight in alloxan induced.
Comparison of streptozotocin and alloxaninduced diabetes in. Alloxan is selectively toxic to insulinproducing pancreatic beta cells because it preferentially accumulates in beta cells through uptake via the glut2 glucose transporter. Wheatgrass has many characteristics desirable for health. The protective effect of cordymin, a peptide purified from. Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. This study was undertaken to evaluate the hypoglycaemic effect of this drug in alloxan induced diabetic rats.
The mechanism of alloxan and streptozotocin action in b. Diabetes induced by coxsackie virus infection is a direct. Diabetes was induced with administration of alloxan, 150 mgkg body weight i. We report herein the findings of this research work. Sir, we read with interest the article titled anomalies in alloxan induced diabetic model. Zaman institute of molecular biology and biotechnology, the university of lahore. Lakshmi bhavani department of botany, university college of science saifabad, osmania university, hyderabad 500004, telangana, india. Research article alloxan induced diabetes mellitus in. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Significant decrease in blood glucose sugar level was observed in comparison to the standard.
The mechanisms of alloxan and streptozotocininduced. How to induce diabetes in rats with alloxan diabetestalk. Alloxaninduced diabetes is a form of insulin dependent diabetes mellitus that occurs as. The extracts showed a dosedependent fall in fbg in alloxan induced diabetic rats. Effect of shilajit on blood glucose and lipid profile in alloxan induced diabetic rats n. Anion gap toxicity in alloxan induced type 2 diabetic rats treated with. Frontiers antihyperglycemic and antihyperlipidemic effects. Antihyperlipidemic and antiperoxidative effect of diasulin, a. Rats were divided into three equal groups, control, diabetic non treated and. In alloxan induced diabetic rats by oral administration of extract 100mgkg body weight. Alloxaninduced diabetes mellitus how is alloxaninduced. Antidiabetic study of vitamin b6 on hyperglycaemia induced. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter.
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